Acute effects of exogenous nitric oxide on glucose uptake in skeletal muscleof normoglycaemic and diabetic rats.
Donovan McGrowder, Dalip Ragoobirsingh, Paul Brown
Med Sci Monit 2006; 12(1): BR28-35
Available online: 2005-12-22
Background: The role of nitric oxide (NO) in the modulation of basal andinsulin-stimulated glucose uptake remains controversial. The aim of this study was to investigate therole of NO released from its donors, S-nitrosoglutathione (GSNO) and S-nitroso-N-acetylpenicillamine(SNAP), on glucose uptake in skeletal muscle of normoglyceamic and type 2 diabetic rats. Material/Methods:Skeletal muscle strips of type 2 diabetic and normoglycaemic Sprague-Dawley rats were incubated withor without various concentrations (200 microM, 500 microM, 1000 microM, 10 mM & 20 mM) of SNAP or GSNOin the presence or absence of insulin (100 nM or 10 microM). The associated radioactivity was determinedby liquid scintillation counting in a Beckman LS6000 scintillation counter programmed for dual-channelcounting Results: SNAP and GSNO at 1000 microM significantly elevated basal and insulin-stimulated 2-deoxyglucoseuptake (P
Keywords: Diabetes Mellitus, Experimental, Blood Glucose - metabolism, Animals, Glucose - metabolism, Insulin - metabolism, Muscle, Skeletal - metabolism, Nitric Oxide - metabolism, Nitric Oxide Donors - metabolism, Rats, Rats, Sprague-Dawley, S-Nitroso-N-Acetylpenicillamine - metabolism, S-Nitrosoglutathione - metabolism