22 December 2005
Macrophage may responses to androgen via its receptor.
Kazem Ahmadi, Alan B. McCrudenMed Sci Monit 2006; 12(1): BR15-20 :: ID: 443171
Abstract
Background: Sex hormones have profound effects on immune responses andmay influence the disease which caused by intracellular parasite(Leishmania) and bacterial (tuberculosis)andalso autoimmune disease such as rheumatoid arthritis (RA). It has also been demonstrated that 5α-Dihydrotestosterone(5α-DHT) modulate nitric oxide and cytokine release by macrophages. These effects seem to be exertedby specific receptors for androgen in macrophages. Material/Methods: Protein secretion: The effect of5α-DHT on protein secretion by peritoneal macrophages of NZBBALBc mice was investigated using radiolabelledprotein secretion following SDS-PAGE and Fluorography. Binding Assay: Androgen binding was also investigatedusing an autoradiography method. Peritoneal macrophages were treated with [[sup]3[/sup]H]- 5α-DHT and incubatedfor 2 h before smearing on to microscope slides. Slides were air dried, dipped in Kodak NTB photographicemulsion, sealed in light proof boxes and left at 4 degrees C for 6 weeks. Results: The results showedthat protein secretion by macrophages changed under 5α-DHT treatment. Analysis of the data accordingto quantitation of [[sup]3[/sup]H]-5αDHT binding receptors in fixed-slide mounted cells, identified a highspecific androgen binding at physiological concentration. The receptors had a relatively high affinityfor the 5α-DHT, So that binding affinity was not inhibited in the presence of 100-fold excess ofnon labelled 17-β Estradiol. Conclusions: These results suggest that the immunosuppressive actionexerted by androgen is at least partially achieved through a direct influence on macrophages.
Keywords: Androgens - pharmacology, Autoradiography, Dihydrotestosterone - pharmacology, Estradiol - metabolism, Macrophages, Peritoneal - metabolism, Radioligand Assay, Receptors, Androgen - metabolism
Editorial
01 April 2024 : Editorial
Editorial: Forty Years of Waiting for Prevention and Cure of HIV Infection – Ongoing Challenges and Hopes for Vaccine Development and Overcoming Antiretroviral Drug ResistanceDOI: 10.12659/MSM.944600
Med Sci Monit 2024; 30:e944600
In Press
06 Mar 2024 : Clinical Research
Prevalence and Variation of Medical Comorbidities in Oral Surgery Patients: A Retrospective Study at Jazan ...Med Sci Monit In Press; DOI: 10.12659/MSM.943884
08 Mar 2024 : Clinical Research
Evaluation of Foot Structure in Preschool Children Based on Body MassMed Sci Monit In Press; DOI: 10.12659/MSM.943765
15 Apr 2024 : Laboratory Research
The Role of Copper-Induced M2 Macrophage Polarization in Protecting Cartilage Matrix in OsteoarthritisMed Sci Monit In Press; DOI: 10.12659/MSM.943738
07 Mar 2024 : Clinical Research
Knowledge of and Attitudes Toward Clinical Trials: A Questionnaire-Based Study of 179 Male Third- and Fourt...Med Sci Monit In Press; DOI: 10.12659/MSM.943468
Most Viewed Current Articles
17 Jan 2024 : Review article
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
14 Dec 2022 : Clinical Research
Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990
Med Sci Monit 2022; 28:e937990
16 May 2023 : Clinical Research
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952