01 October 2005
'Hydrolysis of Myelin Basic Protein by IgM and IgA Antibodies From the Sera of Patients with Multiple Sclerosis' - in replyShigemasa Sawada
Med Sci Monit 2005; 11(10): LE16-16 :: ID: 430348
I enjoyed to read Newsletter of Medical Science Monitor Publication which is the August 2005 issue. Especially, I was interested in an article subscribed by Polosukhina DI et al entitled Hydrolysis of myelin basic protein by IgM and IgA antibodies from the sera of patients with multiple sclerosis . They display the generation by the immune systems of individual multiple sclerosis (MS) patients of a variety of polyclonal IgGs, IgMs, and IgAs with different proteolytic properties, which hydrolyze myelin basic protein(MBP), the major protein component of the myelin-proteolipid shell of axons and a well-known MS autoantigen.
It is true that the catalytic activity of the Ig fractions was not due to contamination by enzyme as author described. Although this article proved the rigid criteria as the presence of catalytic activity activity only in the antibodies of patients with autoimmune dieseas and not in healthy donors, as electrophoretic homogeneity of IgMs and IgAs, as complete adsorption of the hMBP-hydrolyzing activity by anti-IgM and anti-IgA Sepharoses, as FPLC gel filtration of the IgMs and IgAs did not lead to a disappearance of the activity and so on, there is no evidence that the Fab fragment, prepared from IgM or IgA by papain treatment, retained the hydrolytic activity to hMBP which was proven by the first article concerning natural abzymes to the VIP-hydrolyzing activity from the sera of paients with asthma . It is more reliable to be able to show that a monoclonal antibody and a Fab fraction of this monoclonal antibody to hMBP has a hMBP-hydrolyzing activity. It is reasonable that the destruction of myelin-proteolipid shell of axons may be due to this the hMBP-hydrolyzing activity by immunoglobulins in the MS patients. Why the autoantibody has only this hydrolyzing activity? Does the natural antibody to just conventional virus hydrolyze an antigen molecule of virus ? It should be analyzed this possibility using the specific monoclonal antibody (Fab fraction) to a virus. What kind of gene involve to this kind of activity ? Anyway, the catalytic natural antibody may be also an important role of natural immune response to prevent the infection as well as the catalytic auto-antibodies from patients with autoimmune disease.
Professor of Medicine, Division of Rheumatology
and Hematology, Nihon University Nerima Hikarigaoka Hospital, Tokyo, Japan,
1.Polosukhinaa DI, Buneva VN, Doronin BM et al: Hydrolysis of myelin basic protein by IgM and IgA antibodies from the sera of patients with multiple sclerosis. Med Sci Monit, 2005; 11(8): BR266–BR272
2.Paul S, volle DJ, Beach CM et al: Catalytic hydrolysis of vasoactive intestinal peptide by human autoantibody. Science, 1989; 244: 1158–61
Keywords: Catalysis, Chromatography, Gel, Hydrolysis, Immunoglobulin A - blood, Immunoglobulin M - blood, Multiple Sclerosis - blood, Myelin Basic Protein - blood
This Week Editorial
13 September 2021 : EditorialEditorial: The First Monoclonal Antibody Vaccine to Prevent Malaria Heralds a New Era of Malaria Vaccines to the Plasmodium falciparum Circumsporozoite Protein (PfCSP)
Med Sci Monit 2021; 27:e934676
15 September 2021 : EditorialEditorial: Autoantibodies to Components of the Immune System, Including Type 1 Interferons, and the Risk of...
Med Sci Monit In Press; DOI: 10.12659/MSM.934766
08 September 2021 : Clinical ResearchAcceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...
Med Sci Monit In Press; DOI: 10.12659/MSM.932788
16 July 2021 : Review articleSilent Hypoxemia in Patients with COVID-19 Pneumonia: A Review
Med Sci Monit In Press; DOI: 10.12659/MSM.930776
15 Sep 2021 : Clinical ResearchPolish Medical Air Rescue Interventions Concerning Pregnant Women in Poland: A 10-year Retrospective Analysis
Med Sci Monit In Press; DOI: 10.12659/MSM.933029
15 Sep 2021 : EditorialEditorial: Autoantibodies to Components of the Immune System, Including Type 1 Interferons, and the Risk of...
Med Sci Monit In Press; DOI: 10.12659/MSM.934766
15 Sep 2021 : Clinical ResearchA Prospective Single-Center Study of the Effects of Repetitive Transcranial Magnetic Stimulation at 2-Week ...
Med Sci Monit In Press; DOI: 10.12659/MSM.933017
20 Mar 2020 : Clinical ResearchSocial Capital and Sleep Quality in Individuals Who Self-Isolated for 14 Days During the Coronavirus Diseas...
Med Sci Monit 2020; 26:e923921
15 Apr 2020 : Clinical ResearchPsychological Impact and Coping Strategies of Frontline Medical Staff in Hunan Between January and March 20...
Med Sci Monit 2020; 26:e924171
05 May 2020 : Review articleAn Evidence Based Perspective on mRNA-SARS-CoV-2 Vaccine Development
Med Sci Monit 2020; 26:e924700