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Nitric oxide increases gene expression of Ca(2+)-ATPase in myocardial and skeletalmuscle sarcoplasmic reticulum: physiological implications.

Igor Yu Malyshev, Nadezhda P. Aymasheva, Eugeny B. Malenyuk, Georgy L. Khaspekov, Eugenia B. Manukhina, Vasak D. Mikoyan, Ludmila N. Kubrina, Antoly F. Vanin

Med Sci Monit 2000; 6(3): BR480-485

ID: 421322

Available online:


The aim of the study was to verify the hypothesis that NO-dependent regulationof sarcoplasmic reticulum Ca(2+)-ATPase (SERCA) gene expression can play an important role in preventionof calcium overload under the influence of detrimental factors. It was shown that 2 hours after the administrationof the NO donor dinitrosyl iron complex (DNIC), the gene expression of myocardial SERCA was increasedby 20% as compared to the control. In skeletal muscles, the maximum increase in SERCA expression wasobserved in 6 hours and amounted to 156% as compared with the initial value. Simultaneously DNIC enhancedthe resistance of isolated heart and the organism as a whole to damaging effects of intracellular calciumoverload induced by post-ischemic reperfusion or vigorous exercise, respectively. The results obtainedconfirm the existence of NO-dependent activation of SERCA expression and the important role of this mechanismin restriction of calcium overload.

Keywords: Animals, Ca(2+)-Transporting ATPase, Calcium, Gene Expression Regulation, Enzymologic, Iron, Muscle, Skeletal, Myocardium, Nitric Oxide, Nitric Oxide Donors, Nitrogen Oxides, Rats, Rats, Wistar, Research Support, Non-U.S. Gov, Sarcoplasmic Reticulum, Time Factors