Effect of novel platinum complexes on survival rate of B16 and Cl S91 melanoma cells and oxidation stress in vitro
Tomasz Drewa, Alina Wozniak, Gerard Drewa, Dorota Olszewska, Bartosz Wozniak, Mariusz Wysocki, Edward Szlyk, Anna Łakomska
Med Sci Monit 2001; 7(4): BR680-686
BACKGROUND: The aim of study was the assessment of two novel platinum complexesand their cytotoxic effect on murine melanoma cells. The evaluation of possible oxidation stress in neoplasticcells coexistent with the effect of these cytostatic agents.
MATERIAL AND METHODS: Two lines of murinemetastatic melanomas: B16 and Cl S91 were selected for the experiments. Two platinum compounds were tested: cis-dichloro(dimethylsulphoxide)(1-beta-D-ribofuranosyl-1,2,4-triazol-3-carboxyamide)platinum (II) calledPt-complex 1 and chloro(dimethylsulphoxide)(1-beta-D-ribofuranosyl-1,2,4-triazol-3-carboxyamide)platinum(II) called Pt-complex 2. Melanoma cells were incubated for 24 hours with the platinum complexes mentionedabove and culture medium at the concentration of 0.01, 0.05 and 0.5 mg/ml. Survival rate of melanomacells, superoxide dismutase (SOD) activity, the concentration of thiobarbituric acid reactive substances(TBARS) and the content of conjugated dienes were estimated.
RESULTS: A number of statistically significantrelationships between analysed parameters were observed. Negative correlation ratios were found betweenincreasing levels of analysed platinum complexes and the survival rate of the cells of both melanomalines as well as between the survival rate of Cl S91 melanoma cells and the level of lipid peroxidationproducts and SOD activity. On the other hand, there was a positive correlation between increasing platinumcomplexes and the level of lipid peroxidation products as well as between the survival rate of B16 melanomacells and SOD activity and the content of CD.
CONCLUSIONS: Oxygen free radicals may play an importantrole in the development of melanoma and cytostatic agents disturb the balance between generation andremoval of reactive oxygen forms in melanoma cells.
Keywords: Superoxide Dismutase, concentration of thiobarbituric acid reactive substances, conjugated dienes, Melanoma, platinum complexes