Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

09 January 2002

Detecting the replication of the hepatitis B virus using the ImmunoMax technique following treatment with interferon-alpha in children with chronic hepatitis.

Jacek Wysocki, Aldona Kasprzak, Maciej Zabel, Joanna Surdyk-Zasada

Med Sci Monit 2002; 8(1): PR1-7 :: ID: 420958


BACKGROUND: Children with HBV in Poland are treated with preparations ofinterferon-alpha (IFN-alpha). The continuing lack of complete response to this type of anti-viral therapyremains to be explained. The application of cell biology techniques to identify the viral componentsin situ makes it possible to clarify the association between the distribution of the virus and morphologicalinjury to the liver, the immune response of the host, and clinical symptoms in the natural course ofinfection. Our study was intended to evaluate HBV expression in liver biopsies taken an average of twoyears after completion of IFN-a therapy in 10 children with serological markers of persistent HBV infection.MATERIAL/METHODS: For the immunocytochemical detection of HBcAg and for the hybridocytochemical detectionof HBV-DNA, the avidin-biotin-peroxidase (ABC) technique was employed, as well as classical in situ hybridization,both additionally amplified using the ImmunoMax technique. HBcAg and HBV-DNA levels were estimated usinga semiquantitative technique. RESULTS: Our study demonstrated persistent active replication of HBV inthe liver in all examined children. A mixed pattern of HBcAg localization prevailed (noted in cell nuclei,cytoplasm and cell membranes) with a somewhat lower proportion of involved cells and a more evident membranelocalization of HBcAg, as compared to results obtained before treatment. HBV-DNA was observed in thecytoplasm of a fraction of hepatocytes similar to that noted before therapy. CONCLUSIONS: The ImmunoMaxtechnique was found to be highly suitable for in situ monitoring of HBV replication after terminationof IFN-a treatment. Children with focal distribution of HBcAg and HBV-DNA have the potential for earliereradication of the virus from their livers.

Keywords: Adolescent, Avidin, Biotin, Child, Cytological Techniques, Follow-Up Studies, Hepatitis B Core Antigens, Hepatitis B virus, Hepatitis, Chronic, In Situ Hybridization, Interferon-alpha, Liver, Peroxidase, Time Factors


Weekly Editorial

18 October 2021 : Editorial

Editorial: Global Initiatives Support the Use and Regulation of Digital Health Technology During the COVID-19 Pandemic

Dinah V. Parums
Science Editor, Medical Science Monitor, International Scientific Information, Inc., Melville, NY, USA

DOI: 10.12659/MSM.935123

Med Sci Monit 2021; 27:e935123


20 October 2021 : Editorial

Editorial: The 2021 European Society of Cardiology (ESC) Guidelines on the Real-World Prevention of Atheros...

Med Sci Monit In Press; DOI: 10.12659/MSM.935172  

11 October 2021 : Clinical Research

Clinical Features and Temporal Lung Radiographic Changes in 25 Patients Recovering from COVID-19 Pneumonia:...

Med Sci Monit In Press; DOI: 10.12659/MSM.933381  

08 September 2021 : Clinical Research

Acceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...

Med Sci Monit In Press; DOI: 10.12659/MSM.932788  

In Press

20 Oct 2021 : Editorial

Editorial: The 2021 European Society of Cardiology (ESC) Guidelines on the Real-World Prevention of Atheros...

Med Sci Monit In Press; DOI: 10.12659/MSM.935172  

19 Oct 2021 : Review article

Role of NLRP3 Inflammasome in Myocardial Ischemia-Reperfusion Injury and Ventricular Remodeling

Med Sci Monit In Press; DOI: 10.12659/MSM.934255  

18 Oct 2021 : Clinical Research

Adverse Side Effects Associated with Corticosteroid Therapy: A Study in 39 Patients with Generalized Myasth...

Med Sci Monit In Press; DOI: 10.12659/MSM.933296  

13 Oct 2021 : Clinical Research

Endovascular Repair of Thoracic Aorta Injury: 17 Years of Single-Center Experience

Med Sci Monit In Press; DOI: 10.12659/MSM.934479  

Most Viewed Current Articles

20 Mar 2020 : Clinical Research

Social Capital and Sleep Quality in Individuals Who Self-Isolated for 14 Days During the Coronavirus Diseas...

DOI :10.12659/MSM.923921

Med Sci Monit 2020; 26:e923921

15 Apr 2020 : Clinical Research

Psychological Impact and Coping Strategies of Frontline Medical Staff in Hunan Between January and March 20...

DOI :10.12659/MSM.924171

Med Sci Monit 2020; 26:e924171

05 May 2020 : Review article

An Evidence Based Perspective on mRNA-SARS-CoV-2 Vaccine Development

DOI :10.12659/MSM.924700

Med Sci Monit 2020; 26:e924700

26 Apr 2020 : Clinical Research

Comparison of Prevalence and Associated Factors of Anxiety and Depression Among People Affected by versus P...

DOI :10.12659/MSM.924609

Med Sci Monit 2020; 26:e924609

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750