22 February 2002
High affinity glutamate uptake in rat brain slices at experimental crush syndrome.
Garegin Marouchyan, Guevork Kevorkian, Varduhi Knaryan, Ludmila ArakelyanMed Sci Monit 2002; 8(2): BR75-79 :: ID: 420943
Abstract
BACKGROUND: The pathogenesis of crush syndrome is severe traumatic damageto the organism accompanied by shock and stress, acute toxemia, etc. Glutamate has been shown to be implicatedin excitotoxic neural death in various clinical settings. The high affinity glutamate uptake processplays a key role in normal synaptic transmission. The goal of our research was to determine the levelof glutamate uptake in brain tissue slices after incorporation of [(14)C-U]glutamate. MATERIAL/METHODS:An experimental model of crush syndrome in Wistar male rats was caused by 2 hours of compression of femoralsoft tissues using a special press with a force of 100 kg/1 kg of animal weight. Slices were preparedand glutamate uptake was assayed immediately after compression, and again at 2, 24, and 48 hours of decompression.RESULTS: The level of glutamate uptake in cerebral cortex and hypothalamus slices immediately after compressionwas reduced by 24% (p<0.05) and 31% (p<0.005) respectively. Glutamate uptake in cerebral cortex and hypothalamus was nearly at the same level as the intact group of animals (without compression). In cerebellum slices, glutamate uptake also increased, and in fact exceeded the level in the intact group by 17% (p<0.025). The level of glutamate uptake decreased in cerebral cortex, hypothalamus, and cerebellum slices by 45% (p<0.001), 28% (p<0.001,) and 25% (p<0.001) respectively, compared to the intact group of animals.
Conclusions: Experimental crush syndrome promotes the reduction of the glutamate uptake level in brain tissue slices, such that cerebral cortex > hypothalamus > cerebellum.
Keywords: Brain, crush syndrome, Glutamic Acid, in vitro, Rats, Wistar
Editorial
01 March 2024 : Editorial
Editorial: First Regulatory Approvals for CRISPR-Cas9 Therapeutic Gene Editing for Sickle Cell Disease and Transfusion-Dependent β-ThalassemiaDOI: 10.12659/MSM.944204
Med Sci Monit 2024; 30:e944204
In Press
21 Feb 2024 : Clinical Research
Potential Value of HSP90α in Prognosis of Triple-Negative Breast CancerMed Sci Monit In Press; DOI: 10.12659/MSM.943049
22 Feb 2024 : Review article
Differentiation of Native Vertebral Osteomyelitis: A Comprehensive Review of Imaging Techniques and Future ...Med Sci Monit In Press; DOI: 10.12659/MSM.943168
23 Feb 2024 : Clinical Research
A Study of 60 Patients with Low Back Pain to Compare Outcomes Following Magnetotherapy, Ultrasound, Laser, ...Med Sci Monit In Press; DOI: 10.12659/MSM.943732
26 Feb 2024 : Clinical Research
Predictive Value of Combined HbA1c and Neutrophil-to-Lymphocyte Ratio for Diabetic Peripheral Neuropathy in...Med Sci Monit In Press; DOI: 10.12659/MSM.942509
Most Viewed Current Articles
17 Jan 2024 : Review article
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
16 May 2023 : Clinical Research
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387
14 Dec 2022 : Clinical Research
Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990
Med Sci Monit 2022; 28:e937990
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952