01 August 2005
NO(2)-induced acute and chronic lung injury cause imbalance of glutathionemetabolism in type II pneumocytes.
Renate Hochscheid, Ute Schuchmann, Elke Kotte, Stephan Kranz, Sven Heinrichs, Bernd MullerMed Sci Monit 2005; 11(8): BR273-279 :: ID: 200945
Abstract
Background: During inspiration the lung is exposed to numerous oxidantsand therefore has developed a system of antioxidant defense. This organ, besides the liver, is the majorsource of glutathione (GSH) metabolism, from which type II pneumocytes are metabolically the most activecells. Material/Methods: To analyze oxidative stress, rats were exposed to air (control) or to 10 ppmnitrogen dioxide (NO(2)) for 3 and 20 days to induce acute and chronic lung injury. As measure of oxidativestress, GSH/GSSG ratios in blood, bronchoalveolar lavage (BAL) and type II pneumocytes were determined.Lipid peroxidation (LPO) was also measured in type II cells. To investigate the basis of these observations,GSH metabolism in type II pneumocytes was studied, analyzing mRNA expression of g-glutamylcystein synthetase(gamma-GCS), glutathione synthetase (GS), gamma-glutamyltranspeptidase (gamma-GT), glutathione peroxidases(GPXs) and glutathione reductase (GR). Furthermore, enzyme activities of GPX and GR were determined.Results: In acute and chronic lung injury the GSH/GSSG ratio was reduced in blood and BAL, but therewas no change in type II pneumocytes. LPO in type II cells was only reduced in acute lung injury. Inboth kinds of lung injury mRNA expression of gamma-GCS, GS and GPX3 decreased, while expression of gamma-GTand GR increased. GPX4 mRNA expression decreased in acute lung injury and increased in the chronic state.Enzyme activity of GPX and GR was generally increased in lung injury. Conclusions: In NO(2) induced acuteand chronic lung injury, GSH metabolism is imbalanced.
Keywords: Acute Disease, Chronic Disease, Enzymes - genetics, Glutathione - biosynthesis, Glutathione - metabolism, Glutathione Peroxidase - metabolism, Lung - drug effects, Lung - injuries, Lung - metabolism, Lung - pathology, Nitrogen Dioxide - toxicity, Oxidation-Reduction - drug effects, Oxidative Stress - drug effects, RNA, Messenger - genetics, RNA, Messenger - metabolism, Rats, Acute Disease, Chronic Disease, Enzymes - genetics, Glutathione - metabolism, Glutathione Peroxidase - metabolism, Lung - pathology, Lung Injury, Nitrogen Dioxide - toxicity, Oxidation-Reduction - drug effects, Oxidative Stress - drug effects, RNA, Messenger - metabolism, Rats
Editorial
01 May 2022 : Editorial
Editorial: Cardiovascular Complications at One Year After SARS-CoV-2 Infection are Independent of Underlying Cardiovascular Risk Factors or Severity of COVID-19DOI: 10.12659/MSM.937048
Med Sci Monit 2022; 28:e937048
SARS-CoV-2/COVID-19
04 May 2022 : Clinical Research
Effects of Wearing Face Masks on Exercise Capacity and Ventilatory Anaerobic Threshold in Healthy Subjects ...Med Sci Monit In Press; DOI: 10.12659/MSM.936069
22 April 2022 : Clinical Research
Factors Associated with Falls During Hospitalization for Coronavirus Disease 2019 (COVID-19)Med Sci Monit In Press; DOI: 10.12659/MSM.936547
27 April 2022 : Meta-Analysis
Effect of the COVID-19 Pandemic on Serum Vitamin D Levels in People Under Age 18 Years: A Systematic Review...Med Sci Monit In Press; DOI: 10.12659/MSM.935823
06 May 2022 : Clinical Research
COVID-19: Another Cause of Dental Anxiety?Med Sci Monit 2022; 28:e936535
In Press
19 May 2022 : Clinical Research
Association Between Serum Homocysteine Levels and Severity of Diabetic Kidney Disease in 489 Patients with ...Med Sci Monit In Press; DOI: 10.12659/MSM.936323
18 May 2022 : Clinical Research
Physical and Psychosocial Concept Domains Related to Health-Related Quality of Life (HRQL) in 50 Girls and ...Med Sci Monit In Press; DOI: 10.12659/MSM.936801
18 May 2022 : Clinical Research
Association Between Variants of the Mannose-Binding Lectin 2 Gene and Susceptibility to Sepsis in the Haina...Med Sci Monit In Press; DOI: 10.12659/MSM.936134
17 May 2022 : Clinical Research
Clinical Application of C-TIRADS Category and Contrast-Enhanced Ultrasound in Differential Diagnosis of Sol...Med Sci Monit In Press; DOI: 10.12659/MSM.936368
Most Viewed Current Articles
30 Dec 2021 : Clinical Research
Retrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...DOI :10.12659/MSM.935379
Med Sci Monit 2021; 27:e935379
08 Mar 2022 : Review article
A Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...DOI :10.12659/MSM.936292
Med Sci Monit 2022; 28:e936292
01 Nov 2020 : Review article
Long-Term Respiratory and Neurological Sequelae of COVID-19DOI :10.12659/MSM.928996
Med Sci Monit 2020; 26:e928996
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952