Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

01 July 2005 : Extended abstract from EFAS 2011  

Apoptotic efficacy and inhibitory effect of dexamethasone on matrix metalloproteinase.

Farshid Saadat, Mohammad Reza Khorramizadeh, Abbas Mirshafiey

Med Sci Monit 2005; 11(7): BR253-257 :: ID: 16978

Abstract

Background: Background: There is now accumulating evidence that matrixmetalloproteinases and apoptosis may play an important role in inflammation processes. This study wasundertaken to determine the effect of dexamethasone on apoptosis and matrix metalloproteinase 2 (MMP-2)activity compared with two nonsteroidal anti-inflammatory drugs (NSAIDs), piroxicam and diclofenac. Material/Methods:A fibrosarcoma (WEHI-164) cell line was used for evaluating tolerability, MMP-2 activity, and apoptosis.Dexamethasone, piroxicam, and diclofenac were used at concentrations of 10-200 microg/ml in triplicate,two-fold dilutions. MMP-2 activity was assessed using zymography. For assessment of apoptosis, terminaldeoxyribonucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) was used. Results: Cytotoxicityanalysis of dexamethasone showed a greater tolerability than diclofenac at concentrations of 10-100 microg/ml,whereas the cytotoxic effect of dexamethasone and piroxicam were parallel at doses of 5-80 microg/ml.The dose-dependent inhibitory effect of dexamethasone on MMP-2 activity was significantly less than thatof the tested NSAIDs at concentrations of 10-80 microg/ml, while it increased at doses of >100 microg/mlcompared with piroxicam. Moreover, the rate of apoptosis for dexamethasone-treated cells was 20.92%,the values for diclofenac and piroxicam being 78% and 28.15%, respectively. Conclusions: Our findingssuggest that dexamethasone is able to induce apoptosis and suppress MMP-2 activity. Collectively, dexamethasonemight be assumed as an agent which could be recommended for chemopreventive purposes.

Keywords: Dexamethasone - pharmacology, Diclofenac - pharmacology, Matrix Metalloproteinase 2 - antagonists & inhibitors, Piroxicam - pharmacology, Anti-Inflammatory Agents, Non-Steroidal - pharmacology, Antineoplastic Agents - pharmacology, Cell Survival - drug effects, Dexamethasone - pharmacology, Diclofenac - pharmacology, Fibrosarcoma, In Situ Nick-End Labeling, Matrix Metalloproteinase 2 - metabolism, Matrix Metalloproteinase Inhibitors, Piroxicam - pharmacology

Comments

Editorial

01 May 2022 : Editorial  

Editorial: Cardiovascular Complications at One Year After SARS-CoV-2 Infection are Independent of Underlying Cardiovascular Risk Factors or Severity of COVID-19

Dinah V. Parums
Science Editor, Medical Science Monitor, International Scientific Information, Inc., Melville, NY, USA

DOI: 10.12659/MSM.937048

Med Sci Monit 2022; 28:e937048

SARS-CoV-2/COVID-19

24 May 2022 : Clinical Research  

Screening for SARS-CoV-2 Infection in Students at the Medical University of Warsaw, Poland Between November...

Med Sci Monit In Press; DOI: 10.12659/MSM.936962  

04 May 2022 : Clinical Research  

Effects of Wearing Face Masks on Exercise Capacity and Ventilatory Anaerobic Threshold in Healthy Subjects ...

Med Sci Monit In Press; DOI: 10.12659/MSM.936069  

27 May 2022 : Clinical Research  

Factors Associated with Falls During Hospitalization for Coronavirus Disease 2019 (COVID-19)

Med Sci Monit 2022; 28:e936547

In Press

27 May 2022 : Clinical Research  

Prognostic Significance of Preoperative Integrated Liver Inflammatory Score in Patients with Hepatocellular...

Med Sci Monit In Press; DOI: 10.12659/MSM.937005  

25 May 2022 : Review article  

Narrative Review of the Mechanism of Hip Prosthesis Dislocation and Methods to Reduce the Risk of Dislocation

Med Sci Monit In Press; DOI: 10.12659/MSM.935665  

24 May 2022 : Clinical Research  

Remimazolam Tosilate Sedation with Adjuvant Sufentanil in Chinese Patients with Liver Cirrhosis Undergoing ...

Med Sci Monit In Press; DOI: 10.12659/MSM.936580  

24 May 2022 : Clinical Research  

Screening for SARS-CoV-2 Infection in Students at the Medical University of Warsaw, Poland Between November...

Med Sci Monit In Press; DOI: 10.12659/MSM.936962  

Most Viewed Current Articles

30 Dec 2021 : Clinical Research  

Retrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...

DOI :10.12659/MSM.935379

Med Sci Monit 2021; 27:e935379

08 Mar 2022 : Review article  

A Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...

DOI :10.12659/MSM.936292

Med Sci Monit 2022; 28:e936292

01 Nov 2020 : Review article  

Long-Term Respiratory and Neurological Sequelae of COVID-19

DOI :10.12659/MSM.928996

Med Sci Monit 2020; 26:e928996

01 Jan 2022 : Editorial  

Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...

DOI :10.12659/MSM.935952

Med Sci Monit 2022; 28:e935952

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750