Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

02 May 2003

Reg (Regenerating Gene) expression by PARP and NF-kB

H. Okamoto, T. Akiyama, K. Nata, T. Ikeda, N. Shervani, S. Kawaguchi, A. Yamauchi, T. Onogawa, N. Noguchi, S. Takasawa

Med Sci Monit 2003; 9(1): 50- :: ID: 15113

Abstract

In 1981, we proposed that maintenance of the cellular NAD level in b-cells of pancreatic islets is essential for the synthesis and secretion of insulin and for the repair of b-cell injuries, and presented a unifying model for b-cell damage and its prevention (The Okamoto model), in which PARP activation plays an essential role in the depletion of NAD. In 1984, we demonstrated that the administration of PARP inhibitors to 90% depancreatized rats induces islet b-cell regeneration. From the regenerating islet-derived cDNA library we isolated Reg (Regenerating Gene) and demonstrated that Reg protein produced by Reg induces b-cell replication via the Reg receptor and ameliorates experimental diabetes. Recently, we showed that the combined addition of IL-6 and dexamethasone (Dx) induces the Reg gene expression in b-cells and that PARP inhibitors enhance the expression. PARP bound to the cis-element of Reg promoter and formed the active transcriptional DNA/protein complex upon stimulation by IL-6 and Dx. The formation was inhibited depending on the autopoly(ADP-ribosyl)ation of PARP in the complex and by the addition of an antibody against NF-kB. Overexpression of a mutated form of IkB with a serine-to-alanine mutation at residues 32 and 36 as well as the addition of inhibitors for IkB kinase abrogated the Reg gene transcription in response to IL-6/Dx stimulation. Thus, PARP forms the complex for Reg gene transcription with NF-kB. Therefore, the NF-kB activation as well as PARP inhibition could be new therapeutic approaches for b-cell regeneration.

Keywords: pancreatic b-cells, Okamoto model, Necrosis, Reg, Regeneration, NF-kB

Comments

Editorial

01 May 2022 : Editorial  

Editorial: Cardiovascular Complications at One Year After SARS-CoV-2 Infection are Independent of Underlying Cardiovascular Risk Factors or Severity of COVID-19

Dinah V. Parums
Science Editor, Medical Science Monitor, International Scientific Information, Inc., Melville, NY, USA

DOI: 10.12659/MSM.937048

Med Sci Monit 2022; 28:e937048

SARS-CoV-2/COVID-19

24 May 2022 : Clinical Research  

Screening for SARS-CoV-2 Infection in Students at the Medical University of Warsaw, Poland Between November...

Med Sci Monit In Press; DOI: 10.12659/MSM.936962  

04 May 2022 : Clinical Research  

Effects of Wearing Face Masks on Exercise Capacity and Ventilatory Anaerobic Threshold in Healthy Subjects ...

Med Sci Monit In Press; DOI: 10.12659/MSM.936069  

27 May 2022 : Clinical Research  

Factors Associated with Falls During Hospitalization for Coronavirus Disease 2019 (COVID-19)

Med Sci Monit 2022; 28:e936547

In Press

25 May 2022 : Review article  

Narrative Review of the Mechanism of Hip Prosthesis Dislocation and Methods to Reduce the Risk of Dislocation

Med Sci Monit In Press; DOI: 10.12659/MSM.935665  

24 May 2022 : Clinical Research  

Remimazolam Tosilate Sedation with Adjuvant Sufentanil in Chinese Patients with Liver Cirrhosis Undergoing ...

Med Sci Monit In Press; DOI: 10.12659/MSM.936580  

24 May 2022 : Clinical Research  

Screening for SARS-CoV-2 Infection in Students at the Medical University of Warsaw, Poland Between November...

Med Sci Monit In Press; DOI: 10.12659/MSM.936962  

23 May 2022 : Clinical Research  

Fully Automatic Knee Joint Segmentation and Quantitative Analysis for Osteoarthritis from Magnetic Resonanc...

Med Sci Monit In Press; DOI: 10.12659/MSM.936733  

Most Viewed Current Articles

30 Dec 2021 : Clinical Research  

Retrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...

DOI :10.12659/MSM.935379

Med Sci Monit 2021; 27:e935379

08 Mar 2022 : Review article  

A Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...

DOI :10.12659/MSM.936292

Med Sci Monit 2022; 28:e936292

01 Nov 2020 : Review article  

Long-Term Respiratory and Neurological Sequelae of COVID-19

DOI :10.12659/MSM.928996

Med Sci Monit 2020; 26:e928996

01 Jan 2022 : Editorial  

Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...

DOI :10.12659/MSM.935952

Med Sci Monit 2022; 28:e935952

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750