Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

02 May 2003

Poly(ADP-ribosyl)ation, DNA repair and life span

A. Bürkle, S. Beneke, C. Brabeck, A. Leake, R. Meyer, M. L. Muiras, R. Pfeiffer

Med Sci Monit 2003; 9(1): 5-0 :: ID: 14990

Abstract

Poly(ADP-ribosyl)ation is functionally associated with DNA base-excision repair and is a survival factor and negative regulator of genomic instability in cells under low to moderate levels of genotoxic stress [1]. Previously we have described a positive correlation between poly(ADP-ribosyl)ation capacity of mononuclear blood cells with longevity of mammalian species [2]. Our subsequent comparisons of purified recombinant human and rat PARP-1 revealed that this correlation might be explained in part by evolutionary sequence divergence [3]. We have also reported an association of high poly(ADP-ribosyl)ation capacity of lymphoblastoid cell cultures with longevity of human donors [4]. Recently we have asked if the efficiency of single strand-break repair in mononuclear blood cells following gamma-radiation might also be related with mammalian life span. We quantified DNA strand breaks both immediately after irradiation and after a defined postincubation (‘repair’) period, using a recently developed automated version of the fluorescence-detected alkaline unwinding assay. Our data reveal the existence of a highly significant positive correlation between the extent of repair and life span of donor species [5]. In another set of experiments we have investigated the possible effects of L-selegiline on the cellular poly(ADP-ribosyl)ation response. L-selegiline is an anti-Parkinson drug, which irreversibly inhibits monoamine oxidase B (MAO-B) but possesses additional effects, such as neuroprotective activity in vivo and in cultured cells and life span-extension in laboratory animals. Intriguingly, our data show that low concentrations of L-selegiline can potentiate the gamma-radiation-induced poly(ADP-ribose) formation in cultured hamster cells [6], thus strengthening the links between poly(ADP-ribosyl)ation, cytoprotection and mammalian longevity.References: 1.Bürkle A: Cancer Lett, 2001; 163: 1-5 2.Grube K, Bürkle A: Proc Natl Acad Sci USA, 1992; 89: 11759-11763 3.Beneke S, Alvarez-Gonzalez R, Bürkle A: Exp Gerontol, 2000; 35: 989-1002 4.Muiras ML, Müller M, Schächter F, Bürkle A: J Mol Med, 1998; 76: 346-354 5.Pfeiffer R, Leake A, Müller M, Kirkwood TB, Bürkle A: manuscript in preparation. 6.Brabeck C, Pfeiffer R, Leake L, Beneke S, Meyer R, Bürkle A: submitted.

Keywords: PARP-1, DNA strand breaks, DNA Repair, ageing, life span, L-selegiline

Add Comment 0 Comments

Editorial

01 March 2024 : Editorial  

Editorial: First Regulatory Approvals for CRISPR-Cas9 Therapeutic Gene Editing for Sickle Cell Disease and Transfusion-Dependent β-Thalassemia

Dinah V. Parums

DOI: 10.12659/MSM.944204

Med Sci Monit 2024; 30:e944204

0:00

In Press

18 Mar 2024 : Clinical Research  

Sexual Dysfunction in Women After Tibial Fracture: A Retrospective Comparative Study

Med Sci Monit In Press; DOI: 10.12659/MSM.944136  

0:00

21 Feb 2024 : Clinical Research  

Potential Value of HSP90α in Prognosis of Triple-Negative Breast Cancer

Med Sci Monit In Press; DOI: 10.12659/MSM.943049  

22 Feb 2024 : Review article  

Differentiation of Native Vertebral Osteomyelitis: A Comprehensive Review of Imaging Techniques and Future ...

Med Sci Monit In Press; DOI: 10.12659/MSM.943168  

23 Feb 2024 : Clinical Research  

A Study of 60 Patients with Low Back Pain to Compare Outcomes Following Magnetotherapy, Ultrasound, Laser, ...

Med Sci Monit In Press; DOI: 10.12659/MSM.943732  

Most Viewed Current Articles

16 May 2023 : Clinical Research  

Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...

DOI :10.12659/MSM.940387

Med Sci Monit 2023; 29:e940387

0:00

17 Jan 2024 : Review article  

Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron Variant

DOI :10.12659/MSM.942799

Med Sci Monit 2024; 30:e942799

0:00

14 Dec 2022 : Clinical Research  

Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase Levels

DOI :10.12659/MSM.937990

Med Sci Monit 2022; 28:e937990

0:00

01 Jan 2022 : Editorial  

Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...

DOI :10.12659/MSM.935952

Med Sci Monit 2022; 28:e935952

0:00

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750