01 January 2005
The effect of antitumor drugs on oxidative stress in B16 and S91 melanoma cells in vitro.
Alina Woźniak, Gerard Drewa, Bartosz Woźniak, Dietrich O Schachtschabel, Celestyna Mila-Kierzenkowska, Tomasz Drewa, Dorota Olszewska-Słonina, Małgorzata SopońskaMed Sci Monit 2005; 11(1): BR22-29 :: ID: 13867
Abstract
BACKGROUND: This study examined the effect of actinomycin-D (AMD), adriamycin (ADR), cisplatin (Cis-Pt), vincristine (VCR), cytosine arabinoside (Ara-C) and dacarbazine (DTIC) on the survival of B16 and S91 mouse melanoma cells in vitro, and on the concentration of thiobarbituric acid reactive substances (TBARS), the content of conjugated dienes (CD), and the activity of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx). MATERIAL/METHODS: B16 and S91 mice melanoma cells were cultivated in culture medium. After the selected drugs had been added to the culture medium, the viability of the melanoma cells was tested using two methods: the cell count in a phase contrast microscope with inverted optics, and the MTT Test. RESULTS: The survival of melanoma cells after incubation with all cytostatic drugs was significantly lower than in the controls. The concentrations of CD and TBARS and the activity of antioxidant enzymes in melanoma cells after incubation with antitumor drugs were higher than in the controls. The highest concentration of CD and TBARS was shown after incubation with ADR. The highest activity of SOD was noticed after incubation with AMD. The highest activity of CAT was found after incubation with AMD in B16 cells and with Cis-Pt in S91 cells. The highest activity of GPx was found after incubation with Ara-C. CONCLUSIONS: The changes in antioxidant enzyme activity and the concentration of lipid peroxidation products confirm the participation of reactive oxygen species (ROS) in the cytotoxic action of antitumor drugs.
Keywords: Antineoplastic Agents - pharmacology, Antioxidants - metabolism, Catalase - metabolism, Cell Survival - drug effects, Glutathione Peroxidase - metabolism, Lipid Peroxidation - drug effects, Oxidative Stress - drug effects, Superoxide Dismutase - metabolism, Thiobarbituric Acid Reactive Substances - metabolism, Antineoplastic Agents - pharmacology, Antioxidants - metabolism, Catalase - metabolism, Cell Survival - drug effects, Glutathione Peroxidase - metabolism, Lipid Peroxidation - drug effects, Oxidative Stress - drug effects, Superoxide Dismutase - metabolism, Thiobarbituric Acid Reactive Substances - metabolism
Editorial
01 April 2024 : Editorial
Editorial: Forty Years of Waiting for Prevention and Cure of HIV Infection – Ongoing Challenges and Hopes for Vaccine Development and Overcoming Antiretroviral Drug ResistanceDOI: 10.12659/MSM.944600
Med Sci Monit 2024; 30:e944600
In Press
05 Mar 2024 : Clinical Research
Role of Critical Shoulder Angle in Degenerative Type Rotator Cuff Tears: A Turkish Cohort StudyMed Sci Monit In Press; DOI: 10.12659/MSM.943703
06 Mar 2024 : Clinical Research
Comparison of Outcomes between Single-Level and Double-Level Corpectomy in Thoracolumbar Reconstruction: A ...Med Sci Monit In Press; DOI: 10.12659/MSM.943797
21 Mar 2024 : Meta-Analysis
Economic Evaluation of COVID-19 Screening Tests and Surveillance Strategies in Low-Income, Middle-Income, a...Med Sci Monit In Press; DOI: 10.12659/MSM.943863
10 Apr 2024 : Clinical Research
Predicting Acute Cardiovascular Complications in COVID-19: Insights from a Specialized Cardiac Referral Dep...Med Sci Monit In Press; DOI: 10.12659/MSM.942612
Most Viewed Current Articles
17 Jan 2024 : Review article
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
14 Dec 2022 : Clinical Research
Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990
Med Sci Monit 2022; 28:e937990
16 May 2023 : Clinical Research
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952