Douglas P. Olson, David M. Dombkowski, Abigail S. Kelliher, Charlotte Pontillo, David C. Anderson, Frederic I. Preffer
Med Sci Monit 2004; 10(9): BR339-345
Available online: 2004-09-01
BACKGROUND: The assessment of relative antigen density on T cell subsets is a feature of antigen expression that is infrequently characterized. Defining phenotypic differences is a first step in understanding associated differences in function. Additionally, a better understanding of T cell heterogeneity may aid in clinical diagnoses. MATERIAL/METHODS: To further elucidate phenotypic differences of T cell subsets, and begin to determine what information relative antigen density contributes to immunology, we analyzed normal human peripheral blood T cells for a variety of immunophenotypic (CD2, CD3, CD4, CD5, CD7, CD8, CD45RA, CD45RO, TCR alpha beta) and light scatter characteristics using 6 color flow cytometry. T-cell leukemia specimens were also analyzed. RESULTS: Our data show that statistically significant immunophenotypic differences exist between subsets of human CD4 and CD8 T cells. Normal T cells express different levels of relative antigen density for some antigens compared to malignant T cells. CONCLUSIONS: Significant differences are seen in relative antigen density for several cell surface markers between CD4+ and CD8+ T cells. Neither donor source nor flow cytometric calibration account for these differences. The data are applied to specimens from patients with T-lineage acute lymphoblastic leukemia to show how antigen density can be used clinically in aiding to diagnose disease. The data presented here can be used to further investigate these cell populations for functional differences.
Keywords: Leukemia, T-Cell - immunology, Receptors, Antigen, T-Cell - chemistry, Adolescent, Adult, Aged, 80 and over, Antigens, CD - metabolism, CD4-Positive T-Lymphocytes - metabolism, CD8-Positive T-Lymphocytes - metabolism, Child, Child, Preschool, Immunophenotyping, Leukemia, T-Cell - immunology, Receptors, Antigen, T-Cell - metabolism, T-Lymphocyte Subsets