06 January 2004
Comparison of two media for sequential culture after IVF and ICSI shows no differences in pregnancy rates: a randomized trialKlaus-Peter Zollner, Ursula Zollner, Minka Schneider, Johannes Dietl, Thomas Steck
Med Sci Monit 2004; 10(1): CR1-7 :: ID: 11561
Background:Optimal culture conditions are crucial for embryonic development and blastocyst formation after in vitro fertilization (IVF) or Intracytoplasmic sperm injection (ICSI). A prospective randomized trial was carried out to compare the effects of two different sequential media (Vitrolife and MediCult) on embryo development and implantation.Material/Methods: 176 couples were included in this prospective randomized study. All couples undergoing the IVF (n=84) or ICSI (n=92) program at the University of Würzburg were randomly assigned to either Vitrolife (n=87) or MediCult (n=89) sequential culture media.Results: The overall median fertilization rate (FR) was 84.0% and was similar in the Vitrolife (FR=87.5%) and MediCult group (FR=80.0%). A mean of 2.7 embryos were replaced into the uterus, not significantly different between both groups. The pronuclear development was significantly enhanced in embryos cultured in Vitrolife than in those cultured in MediCult media (p=0.035). The embryonic development on day 3 was also superior in the Vitrolife group, but only in ICSI cycles (p<0.01). After 5 days of culturing, the blastocyst development was equivalent in both groups. The clinical pregnancy rate was 31.0% per transfer in the Vitrolife group and 28.1% in the MediCult group (not significant).
Conclusions: The results demonstrate that implantation and clinical pregnancy rates with both media are comparable. Embryonic development until day three seemed to be enhanced when the embryos were cultured in Vitrolife media.
Keywords: Adult, Blastocyst - cytology, Culture Media, Embryo Transfer, Embryonic and Fetal Development, Fertilization in Vitro - methods, Pregnancy, Prospective Studies, Sperm Injections, Intracytoplasmic - methods, Zygote - growth & development, Adult, Blastocyst - cytology, Culture Media, Embryo Transfer, Embryonic and Fetal Development, Fertilization in Vitro - methods, Pregnancy, Prospective Studies, Sperm Injections, Intracytoplasmic - methods, Zygote - growth & development
18 October 2021 : EditorialEditorial: Global Initiatives Support the Use and Regulation of Digital Health Technology During the COVID-19 Pandemic
Med Sci Monit 2021; 27:e935123
20 October 2021 : EditorialEditorial: The 2021 European Society of Cardiology (ESC) Guidelines on the Real-World Prevention of Atheros...
Med Sci Monit In Press; DOI: 10.12659/MSM.935172
11 October 2021 : Clinical ResearchClinical Features and Temporal Lung Radiographic Changes in 25 Patients Recovering from COVID-19 Pneumonia:...
Med Sci Monit In Press; DOI: 10.12659/MSM.933381
08 September 2021 : Clinical ResearchAcceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...
Med Sci Monit In Press; DOI: 10.12659/MSM.932788
21 Oct 2021 : Database AnalysisConstruction and Verification of a Hypoxia-Stemness-Based Gene Signature for Risk Stratification in Esophag...
Med Sci Monit In Press; DOI: 10.12659/MSM.934359
21 Oct 2021 : Database AnalysisMS4A1 as a Potential Independent Prognostic Factor of Breast Cancer Related to Lipid Metabolism and Immune ...
Med Sci Monit In Press; DOI: 10.12659/MSM.934597
21 Oct 2021 : Clinical ResearchPredicting Values of Neutrophil-to-Lymphocyte Ratio (NLR), High-Sensitivity C-Reactive Protein (hs-CRP), an...
Med Sci Monit In Press; DOI: 10.12659/MSM.934569
Most Viewed Current Articles
20 Mar 2020 : Clinical ResearchSocial Capital and Sleep Quality in Individuals Who Self-Isolated for 14 Days During the Coronavirus Diseas...
Med Sci Monit 2020; 26:e923921
15 Apr 2020 : Clinical ResearchPsychological Impact and Coping Strategies of Frontline Medical Staff in Hunan Between January and March 20...
Med Sci Monit 2020; 26:e924171
05 May 2020 : Review articleAn Evidence Based Perspective on mRNA-SARS-CoV-2 Vaccine Development
Med Sci Monit 2020; 26:e924700