Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

18 March 2022: Clinical Research

Unveiling the Differences in Biological Properties of Dental Pulp Stem Cells from Normal and Inflamed Pulp: A Comprehensive Comparative Study

Shao-Chen Nie 12ABCDEF* , Kun Yang 3ABCD* , Na-Na Luan 12F , Xiao-Li Lian 2B , Xiao-Hua Dai 2C , Su-Xia Liang 12A* , Ying-Bin Yan 24A*

DOI: 10.12659/MSM.934511

Med Sci Monit 2022; 28:e934511

Figure 7 Angiogenic differentiation induction analysis. The cells were cultured in non-differentiated control medium (−) and angiogenic differentiation medium (+) for 2 weeks. (A) The cells showed a flat, paving-stone like shape after 14-day angiogenic induction (magnification, ×20; scale, 100 μm). (B) DPSCs and iDPSCs could form tubular network-like structure by Matrigel angiogenesis assay (magnification, ×20; scale, 100 μm). (C) several angiogenic lineage-specific genes were detected by real-time PCR. mRNA levels were tested in 5 independent samples. * P<0.05. (D) Expression of CD31 and VEGF in DPSCs and iDPSCs by immunofluorescence staining. All nuclei were stained with DAPI (blue). The cells were stained with antibody to VEGF (green), and CD31 (red). It was confirmed that DPSCs and iDPSCs co-expressed VEGF and CD31. magnification, ×20; scale bar, 100 μm. DPSCs – dental pulp stem cells; iDPSCs – inflammatory dental pulp stem cells; DAPI – 4′,6-diamidino-2-phenylindole. (Figure were created using Adobe Photoshop CS3, Adobe Systems Software Ireland, Ltd.).

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750