Figure 1 Production of anti-inflammatory or anti-catabolic molecules in incubated healthy human blood samples. (A) Interleukin (IL)-1ra production is significantly increased by incubation in glass surfaces (*** P<0.0001). (B) Comparison of the IL-1ra levels in human blood samples incubated for 3.5 h or 24 h, in the presence or absence of agitation (static vs rocking; * P<0.001). (C) Analysis of tissue inhibitor of metalloproteinase (TIMP)-1, TIMP-2, TIMP-3, and TIMP-4 concentrations in human blood samples before (baseline level) and after incubation at 37°C for 24 h. Two-way revealed a statistically significant (* P<0.001, *** P<0.0001) increase in the levels of TIMP-1 and TIMP-2 at 24 h after incubation. The figure was created using ImageJ software version 1.53e; Java 1.8.0_172.