18 October 2021: Lab/In Vitro Research
Salicylate Induced GABAR Internalization by Dopamine D1-Like Receptors Involving Protein Kinase C (PKC) in Spiral Ganglion Neurons
Jiangyuan Qin ABCDEF , Tingjia Wei BCD , Huiying Chen BCD , Xiaoyu Lin BC , Danxue Qin BC , Fangyu Wei BC , Peiqiang Liu BC , Wenhua Ye BC , Jiping Su ADG*DOI: 10.12659/MSM.933278
Med Sci Monit 2021; 27:e933278
Figure 2 GABARγ20 protein expression in the absence or presence of endocytosis inhibitor (dynasore, Dyn). (A) The surface expression of GABARγ2 (normalized to flotillin-1) was significantly decreased by SKF as compared with the control group, and this effect was completely reversed by SCH and Dyn. The total protein expression of GABARγ2 (normalized to βIII-tubulin) in these groups showed no significant difference. (B) The surface expression of GABARγ2 was significantly decreased by SS and SS+SKF as compared with the control group, which was partially reversed by SCH and completely reversed by Dyn. The total protein expression of GABARγ2 in these groups was not significantly different. Data are presented as mean±SD. All experiments n=4, * P<0.05, vs the control group, # P<0.05, vs the SKF group, & P<0.05 vs the SS group, by one-way ANOVA and Tukey test. Odyssey 3.0.23 (LI-COR, USA) and SPSS 25.0 (USA) were used for the creation of the figures.