03 April 2021>: Animal Study
Expression of DUSP12 Reduces Lung Vascular Endothelial Cell Damage in a Murine Model of Lipopolysaccharide-Induced Acute Lung Injury via the Apoptosis Signal-Regulating Kinase 1 (ASK1)-Jun N-Terminal Kinase Activation (JNK) PathwayZhao Hui 1BF , Huang Jie 2CD , Guo-Hua Fan 2ABCDEFG*
Med Sci Monit 2021; 27:e930429
Figure 6 DUSP12 affected ASK1-JNK signaling. (A, B) MLECs were transfected with DUSP12 siRNA and treated with LPS. (A, B) Protein levels of P-ASK1, JNK1/2, T-ASK1, and T-JNK1/2 in MLECs transfected with siDUSP12 (* P<0.05 vs ScRNA/PBS; # P<0.05 vs ScRNA/LPS). (C, D) MLECs were transfected with Ad-DUSP12 and treated with LPS. A and B. Protein levels of P-ASK1, JNK1/2, T-ASK1, and T-JNK1/2 in MLECs transfected with AdDUSP12 (* P<0.05 vs Ad-NC/PBS; # P<0.05 vs Ad-NC/LPS). (E) Co-IP analysis of DUSP12 and ASK1 in MLECs. All in vitro studies were repeated 3 times independently.