03 April 2021>: Animal Study
Expression of DUSP12 Reduces Lung Vascular Endothelial Cell Damage in a Murine Model of Lipopolysaccharide-Induced Acute Lung Injury via the Apoptosis Signal-Regulating Kinase 1 (ASK1)-Jun N-Terminal Kinase Activation (JNK) PathwayZhao Hui 1BF , Huang Jie 2CD , Guo-Hua Fan 2ABCDEFG*
Med Sci Monit 2021; 27:e930429
Figure 5 DUSP12 regulated oxidative stress. (A, B) MLECs were transfected with Ad-DUSP12 and treated with LPS. (C, D) MLECs were transfected with DUSP12 siRNA and treated with LPS. (A, C) Reactive oxygen species (ROS) level, malondialdehyde (MDA) level, and nicotinamide adenine dinucleotide phosphate (NADPH) activity in MLECs. (B, D) Superoxide dismutase 2 (SOD2) and glutathione peroxidases (Gpx) activities in MLECs (* P<0.05 vs Ad-NC/PBS or ScRNA/PBS; # P<0.05 vs Ad-NC/LPS or ScRNA/LPS). All in vitro studies were repeated 3 times independently.