03 April 2021>: Animal Study
Expression of DUSP12 Reduces Lung Vascular Endothelial Cell Damage in a Murine Model of Lipopolysaccharide-Induced Acute Lung Injury via the Apoptosis Signal-Regulating Kinase 1 (ASK1)-Jun N-Terminal Kinase Activation (JNK) PathwayZhao Hui 1BF , Huang Jie 2CD , Guo-Hua Fan 2ABCDEFG*
Med Sci Monit 2021; 27:e930429
Figure 3 DUSP12 suppressed LPS-induced pulmonary endothelial cell injury. (A) Protein level of DUSP12 in murine lung vascular endothelial cells (MLECs) treated with LPS. (B–F) MLECs were transfected with Ad-DUSP12 and treated with LPS. B. Protein level of DUSP12 in MLECs transfected with Ad-DUSP12. (C) mRNA levels of proinflammatory factors. (D) ELISA measurement of proinflammatory factors. (E) TUNEL staining (magnification: 200). (F) Caspase3 activity (* P<0.05 vs Ad-NC/PBS; # P<0.05 vs Ad-NC/LPS). All in vitro studies were repeated 3 times independently.