03 April 2021>: Animal Study
Expression of DUSP12 Reduces Lung Vascular Endothelial Cell Damage in a Murine Model of Lipopolysaccharide-Induced Acute Lung Injury via the Apoptosis Signal-Regulating Kinase 1 (ASK1)-Jun N-Terminal Kinase Activation (JNK) PathwayZhao Hui 1BF , Huang Jie 2CD , Guo-Hua Fan 2ABCDEFG*
Med Sci Monit 2021; 27:e930429
Figure 2 DUSP12 knockdown aggravated LPS-induced lung injury in mice. (A) Protein level of DUSP12 in mouse lung tissue 42 h after Ad-shDUSP12 injection (n=6). (B) Immunofluorescence staining of CD31 and DUSP12 after Ad-shDUSP12 injection (n=5, magnification: 400). (C) H&E staining (n=5, magnification: 200). (D) Lung wet weight/dry weight ratio (W/D ratio) (n=10). (E) Lung injury score (n=6). (F) Proinflammatory factor levels in alveolar lavage fluid as measured by ELISA (n=6) (* P<0.05 vs ScRNA/NS; # P<0.05 vs ScRNA/LPS).