01 June 2021>: Animal Study
Post-Treatment Sevoflurane Protects Against Hypoxic-Ischemic Brain Injury in Neonatal Rats by Downregulating Histone Methyltransferase G9a and Upregulating Nuclear Factor Erythroid 2-Related Factor 2 (NRF2)HuaiMing Wang 1A , YiQuan Xu 1B , Shuying Zhu 1E , XueMing Li 2F , HongWei Zhang 1G*
Med Sci Monit 2021; 27:e930042
Figure 5 Silencing NRF2 reverses the alleviating effect of sevoflurane post-treatment on HIBI neonatal rats. (A) NRF2 protein level in cells of each group was determined by western blot assay. (B) ELISA was utilized to measure the levels of inflammatory factors (TNF-α, IL-1β, IL-6, and IL-8) in cells of each group. (C) Flow cytometry was carried out to observe apoptosis in cells of each group. The data are expressed as the mean±standard deviation. The data among groups were analyzed by one-way ANOVA, followed by Tukey’s multiple comparisons test. * P<0.05.