01 June 2021>: Animal Study
Post-Treatment Sevoflurane Protects Against Hypoxic-Ischemic Brain Injury in Neonatal Rats by Downregulating Histone Methyltransferase G9a and Upregulating Nuclear Factor Erythroid 2-Related Factor 2 (NRF2)HuaiMing Wang 1A , YiQuan Xu 1B , Shuying Zhu 1E , XueMing Li 2F , HongWei Zhang 1G*
Med Sci Monit 2021; 27:e930042
Figure 4 Sevoflurane post-treatment reduces the levels of G9a and H3K9me2 in OGD-induced PC12 cells to alleviate OGD-induced cell damage. (A) The protein levels of G9a and H3K9me2 in cells of each group were detected by western blot analysis. (B) ELISA was utilized to measure the levels of inflammatory factors (TNF-α, IL-1β, IL-6, and IL-8) in cells of each group. (C) Flow cytometry was performed to detect the apoptosis rate of cells in each group. The data are expressed as the mean±standard deviation. The data among groups were analyzed by one-way ANOVA, followed by Tukey’s multiple comparisons test. * P<0.05.