21 April 2021>: Animal Study
Short-Term High-Intensity Treadmill Exercise Promotes Ceramide-Dependent Extracellular Vesicle Secretion in the Central Nervous System of MiceRui Zhang 12ABCEF* , Xiaoyan Liang 1BC* , Shi Tang 1BC , Lin Song 1CD , Jing Zhang 1CF , Yifeng Du 1AG*
Med Sci Monit 2021; 27:e929609
Figure 3 Short-term high-intensity exercise increased the levels of EV biomarkers in the mouse brain. (A) Western blot shows immunoreactivity for TSG101, HSC70 and CD63 from mice sacrificed before exercise (control), immediately after exercise (0 min) and 90 min after exercise (90 min). (B) The TSG101 level showed a significant increase after exercise (p<0.01) and almost returned to baseline within 90 min (P<0.05). (C) The level of CD63 in brain tissue showed no significant changes (P>0.05). (D) The HSC70 level was higher after exercise than before exercise (P<0.01) and almost returned to baseline within 90 min (P<0.05). (E, F) NTA of EVs from the brain tissues of mice sacrificed before exercise (control), immediately after exercise (0 min) and 90 min after exercise (90 min) is shown. The concentration of EVs (E) and the mean diameter of EVs (F) in the brain tissues were determined; n=8/group. Data are expressed as the mean±SD, ** P<0.01 using one-way ANOVA with the Bonferroni post-test.