19 April 2021: Lab/In Vitro Research
Loss of Speckle-Type POZ Protein Promotes Prostate Cancer Cell Migration and Invasion Through Upregulation of MCP-1
Junlin Shi 12ABCDEFG , Ji Cao 3AG , Xiaomei Lu 12B , Langlin Fan 12B , Hongwei Guo 12G , Jiejun Fu 12ABCDEFG*DOI: 10.12659/MSM.929199
Med Sci Monit 2021; 27:e929199
Figure 3 The migration and invasion of sgSPOP-1 and sgSPOP-2 cells in response to their CM. (A) A total of 5×104 sgSPOP-1 cells were seeded to test for migration and invasion ability. Lane 1, RMPI 1640; Lane 2, sgSPOP-1 CM; Lane 3, sgSPOP-1 CM with anti-MCP-1 (2 μg/ml). (B) A total of 5×104 sgSPOP-2 cells were seeded to test for migration and invasion ability. Lane 1, RMPI 1640; Lane 2, sgSPOP-2 CM; Lane 3, sgSPOP-2 CM with anti-MCP-1 (2 μg/ml). The results are presented as the mean±SD of 3 independent experiments; * P<0.05, ** P<0.01. CM – conditioned medium.