19 April 2021>: Lab/In Vitro Research
Loss of Speckle-Type POZ Protein Promotes Prostate Cancer Cell Migration and Invasion Through Upregulation of MCP-1
Junlin Shi 12ABCDEFG , Ji Cao 3AG , Xiaomei Lu 12B , Langlin Fan 12B , Hongwei Guo 12G , Jiejun Fu 12ABCDEFG*DOI: 10.12659/MSM.929199
Med Sci Monit 2021; 27:e929199
Figure 2 The difference in soluble factors in the conditioned media of sgSPOP-1 cells, sgSPOP-2 cells, and control cells, as detected by chemokine antibody arrays and verified by ELISA and qRT-PCR. (A) The difference in expressed chemokines in the conditioned media of sgSPOP-1 cells, sgSPOP-2 cells, and control cells, as detected through chemokine antibody array. (B) ELISA was used to verify the MCP-1 expression level in the conditioned medium. The results are presented as the mean±SD of 3 independent experiments; ** P<0.01. (C) qRT-PCR measurement of the MCP-1 mRNA expression levels. The results are presented as the mean±SD of 3 independent experiments; * P<0.05, ** P<0.01.