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19 April 2021: Lab/In Vitro Research

Loss of Speckle-Type POZ Protein Promotes Prostate Cancer Cell Migration and Invasion Through Upregulation of MCP-1

Junlin Shi 12ABCDEFG , Ji Cao 3AG , Xiaomei Lu 12B , Langlin Fan 12B , Hongwei Guo 12G , Jiejun Fu 12ABCDEFG*

DOI: 10.12659/MSM.929199

Med Sci Monit 2021; 27:e929199

Figure 1 SPOP knockout promotes C4-2 cell migration and invasion. (A) Photomicrograph of sgSPOP-1 and sgSPOP-2 compared with parent cell line C4-2 (control). (B) Western blotting with anti-SPOP antibody confirms the presence of SPOP in control C4-2 cells and absence in both sgSPOP-1 and sgSPOP-2 cells. (C) A transwell assay was used to explore cell migration and invasion ability in sgSPOP-1, sgSPOP-2, and control cells. The results are presented as the mean±SD of 3 independent replicates; * P<0.05, ** P<0.01. (D) The migration ability of sgSPOP-1 cells, sgSPOP-2 cells, and control cells was investigated using a scratch wound-healing assay. The results are shown as the mean±SD of 3 independent replicates; * P<0.05, ** P<0.01.

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750