27 December 2020: Database Analysis
Expression of the Topoisomerase II Alpha (TOP2A) Gene in Lung Adenocarcinoma Cells and the Association with Patient Outcomes
Xiaomei Du 1AEFG , Zhiwen Xue 1BF , Jianning Lv 1BC , Heidou Wang 2ACG*DOI: 10.12659/MSM.929120
Med Sci Monit 2020; 26:e929120
Figure 6 Measurement of relevant indexes after low expression of TOP2A in A549 cells. (A) Cell viability detection by MTT assay. Cell viability of siA549 was significantly lower than that of siControl (* P<0.01). (B) Cell apoptosis detection by flow cytometry. Cell apoptosis of siA549 was obviously higher than that of siControl (* P<0.01). (C) Detection of TOP2A mRNA and protein expressions in siA549 and siControl cells by RT-PCR and Western blot. (D) Detection of proteins related to ERK/JNK/p-P38/CHOP signaling pathway using Western blot.