27 February 2021>: Lab/In Vitro Research
Collagen Type X Alpha 1 (COL10A1) Contributes to Cell Proliferation, Migration, and Invasion by Targeting Prolyl 4-Hydroxylase Beta Polypeptide (P4HB) in Breast Cancer
Weibin Yang ABCDEF* , Xuan Wu ABCEF , Fan Zhou ABCEDOI: 10.12659/MSM.928919
Med Sci Monit 2021; 27:e928919
Figure 7 Silencing of P4HB disrupted the effect of COL10A1 overexpression in the progression of breast cancer. (A). Western blot analysis was performed to detect the transfection efficiency in MCF-7 cells transfected with si-P4HB-1 or si-P4HB-2. (B). RT-qPCR analysis was performed to detect the transfection efficiency in MCF-7 cells transfected with si-P4HB-1 or si-P4HB-2. (C). CCK-8 assay was performed to explore the effect of silenced P4HB on the enhanced cell viability induced by overexpressed COL10A1. (D). Colony formation assay was used to explore the effect of silenced P4HB on the elevated clone-forming ability induced by overexpressed COL10A1. (E, G). Wound healing assay was used to examine the effect of silenced P4HB on the enhanced cell migration ability induced by overexpressed COL10A1. (F, H). Transwell assay was used to examine the effect of silenced P4HB on the enhanced cell invasion ability induced by overexpressed COL10A1. * P<0.05, ** P<0.01, *** P<0.001.