27 February 2021: Lab/In Vitro Research
Collagen Type X Alpha 1 (COL10A1) Contributes to Cell Proliferation, Migration, and Invasion by Targeting Prolyl 4-Hydroxylase Beta Polypeptide (P4HB) in Breast Cancer
Weibin Yang ABCDEF* , Xuan Wu ABCEF , Fan Zhou ABCEDOI: 10.12659/MSM.928919
Med Sci Monit 2021; 27:e928919
Figure 4 Downregulation of COL10A1 suppressed cell proliferation and clone-forming abilities of breast cancer cells. (A) Western blot analysis was performed to detect the transfection efficiency in MCF-7 cells which were transfected with shRNA-COL10A1-1 or shRNA-COL10A1-2. (B) RT-qPCR analysis was performed to detect the transfection efficiency in MCF-7 cells which were transfected with shRNA-COL10A1-1 or shRNA-COL10A1-2. (C) CCK-8 assay was applied to explore the effect of silenced COL10A1 on cell viability of MCF-7. (D) Colony formation assay was applied to explore the effect of silenced COL10A1 on clone-forming ability of MCF-7. (E) Western blot analysis was performed to detect the transfection efficiency in MDA-MB-231 cells which were transfected with shRNA-COL10A1-1 or shRNA-COL10A1-2. (F) RT-qPCR analysis was performed to detect the transfection efficiency in MDA-MB-231 cells which were transfected with shRNA-COL10A1-1 or shRNA-COL10A1-2. (G) CCK-8 assay was applied to explore the effect of silenced COL10A1 on cell viability of MDA-MB-231. (H). Colony formation assay was used to explore the effect of silenced COL10A1 on clone-forming ability of MDA-MB-231. * P<0.05, ** P<0.01, *** P<0.001.