27 January 2021>: Lab/In Vitro ResearchQingxin Fan 12BCDEF , Pengjiao Xi 3ACD , Derun Tian 13AG , Lianqun Jia 4AFG , Yuan Cao 4BDF , Kaixuan Zhan 4BCDF , Tianwei Sun 5BCDG , Yinlong Zhang 6BCD , Qiming Wang 14ABCDEFG*
Med Sci Monit 2021; 27:e928619
Figure 4 Effect of ginsenoside Rb1 (G-Rb1) on browning treated with Compound 3f. (A) Oil Red O staining detected the lipid droplets. Scale bar=100 μm. (B) Optical density values at 490 nm. (C) Lipid droplets’ diameter of ORS was measured by ImageJ. (D) Western blotting results for Ucp-1 and β-catenin, with Gapdh as the internal reference. (E, F) ImageJ quantized the gray value of each protein band. (G) Immunofluorescence was performed to detect the expression of Ucp-1. Scale bar=50 μm. Data are expressed as mean±SD (n=3). * P<0.05, ** P<0.01, *** P<0.001, **** P<0.0001.