27 January 2021>: Lab/In Vitro ResearchQingxin Fan 12BCDEF , Pengjiao Xi 3ACD , Derun Tian 13AG , Lianqun Jia 4AFG , Yuan Cao 4BDF , Kaixuan Zhan 4BCDF , Tianwei Sun 5BCDG , Yinlong Zhang 6BCD , Qiming Wang 14ABCDEFG*
Med Sci Monit 2021; 27:e928619
Figure 2 Effect of ginsenoside Rb1 (G-Rb1; 0, 10, 50, and 100 μM) on the browning of adipocytes. (A) Lipid droplets were measured by Oil Red O staining. Scale bar=100 μm. (B) Lipid content assessing according to the absorbance at 490 nm. (C) ImageJ measured the average diameter of lipid droplets. (D–H) Quantitative real-time polymerase chain reaction measured the mRNA expression of browning-related genes (Cd-137, Cited-1, Tbx-1, Prdm-16, and Ucp-1). (I, J) Western blotting measured the protein expression of Ucp-1. Data are expressed as mean±SD (n=3). * P<0.05, ** P<0.01, *** P<0.001, **** P<0.0001 vs. 0 μM.