22 January 2021>: Animal Study
Evaluation of Demineralized Bone Matrix Particles Delivered by Alginate Hydrogel for a Bone Graft Substitute: An Animal Experimental StudyZhiqiang Yang ABCDEF , Lingfei Xiao BDF , Zhouming Deng AC , Lin Cai AG , Yuanlong Xie ACDEF*
Med Sci Monit 2021; 27:e928617
Figure 2 In vitro cell study. (A) Phenotypic verification of bone marrow mesenchymal stem cells (MSCs) and immunofluorescence against CD44, CD45, and DAPI. (B) 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay of demineralized bone matrix-alginate (ADBM)-100 composites on MSCs and the statistical histogram of optical density (OD) values in the control and ADBM groups. (C) ADBM-100 composite promoted osteogenic differentiation in vitro. Representative images of alkaline phosphatase (ALP) and alizarin red staining (ARS) are displayed. (D) ALP activity. (E) The OD value of calcium deposits detected with ARS. (F) The effects of the ADBM-100 composite on MSCs. RUNX2, OPN, OCN, and collagen I, which are marker genes of osteogenic differentiation, were upregulated.