Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

22 January 2021: Animal Study

Evaluation of Demineralized Bone Matrix Particles Delivered by Alginate Hydrogel for a Bone Graft Substitute: An Animal Experimental Study

Zhiqiang Yang ABCDEF , Lingfei Xiao BDF , Zhouming Deng AC , Lin Cai AG , Yuanlong Xie ACDEF*

DOI: 10.12659/MSM.928617

Med Sci Monit 2021; 27:e928617

Figure 2 In vitro cell study. (A) Phenotypic verification of bone marrow mesenchymal stem cells (MSCs) and immunofluorescence against CD44, CD45, and DAPI. (B) 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay of demineralized bone matrix-alginate (ADBM)-100 composites on MSCs and the statistical histogram of optical density (OD) values in the control and ADBM groups. (C) ADBM-100 composite promoted osteogenic differentiation in vitro. Representative images of alkaline phosphatase (ALP) and alizarin red staining (ARS) are displayed. (D) ALP activity. (E) The OD value of calcium deposits detected with ARS. (F) The effects of the ADBM-100 composite on MSCs. RUNX2, OPN, OCN, and collagen I, which are marker genes of osteogenic differentiation, were upregulated.

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750