12 March 2021>: Lab/In Vitro Research
Citrus Alkaline Extracts Inhibit Senescence of A549 Cells to Alleviate Pulmonary Fibrosis via the β-Catenin/P53 PathwayDi Han 1BDE , Yong Xu 1CE , Wen-Pan Peng 1BD , Fanchao Feng 12AD , Zhichao Wang 1BD , Cheng Gu 1BD , Xianmei Zhou 12AG*
Med Sci Monit 2021; 27:e928547
Figure 1 Adriamycin RD (ARD)-induced senescence in A549 cells. (A, B) The analysis of senescence-associated β-galactosidase staining (SA-β-Gal). The SA-β-Gal-positive cell percentages were scored; the mean±SD of data from at least 3 separate experiments are visualized. Magnification: ×200 # P<0.05 vs Blank. (C, D) Western blot analysis of PDGF-a, TNF-α, PDGF-b, MMP-7, CyclinD1, and CTGF in A549 cells. Each group was assessed in triplicate, and experiments were repeated 3 times. Bar graphs show the relative quantification. # P<0.05, ## P<0.01, and #### P<0.0001 vs Blank.