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09 March 2021: Lab/In Vitro Research

Gasdermin D Influence Mouse Podocytes Against High-Glucose-Induced Inflammation and Apoptosis via the C-Jun N-Terminal Kinase (JNK) Pathway

Huifang Li 1ABCDE , Kunxiao Zhao 2ADF , Ying Li 1ADFG*

DOI: 10.12659/MSM.928411

Med Sci Monit 2021; 27:e928411

Figure 4 Effect of GSDMD knockdown on HG-induced mitochondrial ROS production and p-JNK expression levels in mouse podocytes. (A) Mitochondrial ROS production was measured using a confocal microscope. (B, C) SOD2 activity was evaluated by western blotting. (D, E) The protein activity of JNK and p-JNK was detected by western blotting. NG – normal-glucose; HG – high-glucose; HG+siGSDMD – high-glucose+siGSDMD; HG+SP – high-glucose+SP600125. Data represent the average of 3 measurements. Mean±SD of 3 experiments is shown. (C) * P<0.05 vs NG, # P<0.05 vs HG; (E) * P<0.05 vs HG, # P<0.05 vs HG.

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750