29 May 2021>: Lab/In Vitro Research
Silencing Ribosomal Protein L22 Promotes Proliferation and Migration, and Inhibits Apoptosis of Gastric Cancer Cells by Regulating the Murine Double Minute 2-Protein 53 (MDM2-p53) Signaling PathwayZhenqing Sun 1ABCE* , Zhigang Qiu 1ABCE* , Zhengkun Wang 1DE , Honghui Chi 2DE , Peipei Shan 3CE*
Med Sci Monit 2021; 27:e928375
Figure 5 Ribosomal protein L22 (RPL22) inhibits the proliferation, migration, and invasion of MKN-45 cells through regulation of MDM2-p53 signaling. (A) The protein expression of MDM2 and p53 in MKN-45 cells was detected by a western blot.(B) The optic density (OD450) value of the MKN-45 cells was detected by a CCK-8 assay. (C) The apoptosis rate of the MKN-45 cells was detected by a flow cytometry assay. (D) The relative migration of the MKN-45 cells was detected by a wound healing assay. (E) The relative invasion of the MKN-45 cells was detected by a transwell assay. BLANK – cells without transfection; si-NC – cells transfected with siRNA negative control; si-RPL22-1 – cells transfected with siRNA-RPL22-1; si-RPL22-1+nutlin-3 – cells transfected with siRNA-RPL22-1 and nutlin-3. (A) ** P<0.01 vs control; (B–E) ** P<0.01 vs BLANK; # P<0.05, ## P<0.01 vs si-RPL22-1.