29 May 2021>: Lab/In Vitro Research
Silencing Ribosomal Protein L22 Promotes Proliferation and Migration, and Inhibits Apoptosis of Gastric Cancer Cells by Regulating the Murine Double Minute 2-Protein 53 (MDM2-p53) Signaling PathwayZhenqing Sun 1ABCE* , Zhigang Qiu 1ABCE* , Zhengkun Wang 1DE , Honghui Chi 2DE , Peipei Shan 3CE*
Med Sci Monit 2021; 27:e928375
Figure 2 Ribosomal protein L22 (RPL22) influences the proliferation and apoptosis of gastric cancer cells.(A) The mRNA expression of RPL22 in MKN-45 and MGC-803 cells was detected by a quantitative real-time polymerase chain reaction. (B) The protein expression of RPL22 in MKN-45 and MGC-803 cells was detected by a western blot. (C) The optical density value of the MKN-45 and MGC-803 cells was detected by a CCK-8 assay. (D) The apoptosis rate of the MKN-45 and MGC-803 cells was detected by a flow cytometry assay. BLANK – cells without transfection; pcDNA-NC – cells transfected with pcDNA negative control; pcDNA-RPL22 – cells transfected with pcDNA-RPL22; si-NC – cells transfected with siRNA negative control; si-RPL22-1 – cells transfected with siRNA-RPL22-1; si-RPL22-2 – cells transfected with siRNA-RPL22-2. (A–D) * P<0.05, ** P<0.01, *** P<0.001 vs BLANK; && P<0.01 vs BLANK.