14 January 2021: Lab/In Vitro Research
Mechanical Stress Affects Circadian Rhythm in Skeletal Muscle (C2C12 Myoblasts) by Reducing Per/Cry Gene Expression and Increasing Bmal1 Gene Expression
Mengjia Wang 1BCDEF* , Da Yu 2BCDF* , Lichun Zheng 1BCDF* , Bing Hong 1BCD , Houxuan Li 1BCD , Xiaobei Hu 1BC , Kun Zhang 1BF , Yongbin Mou 1ADEG*DOI: 10.12659/MSM.928359
Med Sci Monit 2021; 27:e928359
Figure 3 Determination of the Per and Cry gene transcriptions and protein expressions in C2C12 myoblasts (n=6). (A) Statistical analysis for Clock gene transcription identified by PCR assay. (B) Statistical analysis for Bmal1 gene transcription of Cry identified by PCR assay. (C) Western blot images for Clock and Bmal1 protein expression. (D) Statistical analysis for Clock expression in C2C12 cells. (E) Statistical analysis for Bmal1 expression in C2C12 cells. * P<0.05 and ** P<0.001 represent differences illustrated in images.