14 January 2021: Lab/In Vitro Research
Mechanical Stress Affects Circadian Rhythm in Skeletal Muscle (C2C12 Myoblasts) by Reducing Per/Cry Gene Expression and Increasing Bmal1 Gene Expression
Mengjia Wang 1BCDEF* , Da Yu 2BCDF* , Lichun Zheng 1BCDF* , Bing Hong 1BCD , Houxuan Li 1BCD , Xiaobei Hu 1BC , Kun Zhang 1BF , Yongbin Mou 1ADEG*DOI: 10.12659/MSM.928359
Med Sci Monit 2021; 27:e928359
Figure 2 Evaluation for the Per and Cry gene transcriptions and protein expressions in C2C12 myoblasts (n=6). (A) Statistical analysis for the gene transcription of Per determining by PCR assay. (B) Statistical analysis for the gene transcription of Cry determining by PCR assay. (C) Western blot images for the Per and Cry protein expression. (D) Statistical analysis for the protein expression of Per in C2C12 cells. (E) Statistical analysis for the protein expression of Cry in C2C12 cells. * P<0.05, ** P<0.01, and *** P<0.001 represents the differences illustrated in images.