15 June 2021>: Animal Study
The Improvement Effect of Sodium Ferulate on the Formation of Pulmonary Fibrosis in Silicosis Mice Through the Neutrophil Alkaline Phosphatase 3 (NALP3)/Transforming Growth Factor-β1 (TGF-β1)/α-Smooth Muscle Actin (α-SMA) Pathway
Jingyin Han AE* , Yangmin Jia BCD , Shujuan Wang BCD , Xiaoyu Gan BCDDOI: 10.12659/MSM.927978
Med Sci Monit 2021; 27:e927978
Figure 2 Fibroblasts were isolated and identified, and sodium ferulate (SF) reduced the expression of fibrosis-related proteins in silicosis mice. (A) Localization and expression of vimentin in cells by immunofluorescence (magnification×200, scale bars=20 μm). (B–I) The effect of SF on the expression of transforming growth factor-β1 (TGF-β1), neutrophil alkaline phosphatase 3 (NALP3), collagen-1, alpha-smooth muscle actin (α-SMA), phosphorylated p38 (p-p38) and p38 proteins, and the ratio of p-p38/p38 in silicosis mice was detected by western blot. Expression levels were normalized with GAPDH. All experiments were performed in triplicate and data are expressed as mean±standard deviation (SD). ** P<0.01, *** P<0.001 vs control 8 weeks; # P<0.05, ## P<0.01, ### P<0.001 vs model 4 weeks+physiological saline 4 weeks.