Figure 2 The effect of cell fibrosis on Treg cells differentiation. (A) Changes of Treg cells proportion in IPF patients was the most obvious. (B) Detection of cytokines in blood of IPF patients. Among them, the changes of IL-4 and IL-10 were the most significant. (C) TGF-beta stimulates pulmonary fibroblasts to obtain myofibroblasts. (TGF-beta concentration: 1 ug/ml) (100×). (D) The purity of CD4+ T cells isolated from peripheral blood by magnetic beads was more than 90%. (E) The proportion of Treg cells decreased significantly after CD4+ T cells co-culture with myofibroblasts for 24 h. ** P<0.01, *** P<0.001.