Figure 2 NEAT1 binds to miR-373 and negatively regulates miR-373 expression. (A) FISH, showing that NEAT1 is mainly located in cytoplasm; (B) subcellular fractionation of trophoblast cells, with U6 as a nuclear control and GAPDH as a cytoplasmic control; (C) binding sites of NEAT1 and miR-373; (D) transfection efficiency of miR-373 mimic/inhibitor determined by RT-qPCR (* p<0.05 by unpaired t test); (E) AT1 or MT-NEAT1 (* p<0.05 by 2-way ANOVA); (F) miR-373 expression in cells transfected with pcDNA-NEAT1 or siRNA-NEAT1, as determined by RT-qPCR (* p<0.05 by 1-way ANOVA). Each figure represents the average of 3 replicates.