Figure 3 DUOX1-regulated wound healing, likely by regulation of reactive oxygen species (ROS). (A, B) Transfected primary fibroblasts with overexpression of human DUOX1 were constructed, and Q-PCR (A) and Western blot (B) were used to detect the efficiency of DUOX1 overexpression. Following pre-transfection of DUOX1 overexpression in primary fibroblasts for 24 h, cells were treated with NAC and ROS inhibitor (10 μM). (C, D) Expression of MDA (C) and SOD (D) were detected in cell supernatants; (E, F) flow cytometry was used to detect ROS and (E) cell apoptosis (F); (G) cell proliferation activity was detected using a CCK-8 kit; (H) expression of NF-κB (nuclear and plasma), collagen I, collagen III, P21, and P16 were detected by Western blotting. * P<0.05, ** P<0.01, *** P<0.001 vs vector or vehicle+vector; ## P<0.01, ### P<0.001 vs vehicle+oeDUOX1.