Figure 2 Knockdown of DUOX1 significantly increased cell proliferation and inhibited the production of reactive oxygen species (ROS). (A) Following primary isolation from wound granulation tissue, fibroblasts (Vimentin-positive) were identified by immunohistochemical staining. (B, C) Primary fibroblasts were transfected with human DUOX1 interferon, and the interference efficiency of DUOX1 was detected by Q-PCR (B) and Western blot (C). Following DUOX1 gene interference in primary fibroblasts, (D) a CCK-8 kit was used to detect cell proliferation; (E, F) biochemical detection of cell supernatant MDA (E) and SOD (F) expression; (G, H) flow detection of ROS and (G) cell apoptosis (H); (I) Western blotting of NF-κB (nuclear and plasma), collagen, collagen III, P21, and P16. ** P<0.01, *** P<0.001 vs siNC. siNC – negative control of DUOX1 interference.