07 September 2020: Lab/In Vitro Research
Reduced Caudal Type Homeobox 2 (CDX2) Promoter Methylation Is Associated with Curcumin’s Suppressive Effects on Epithelial-Mesenchymal Transition in Colorectal Cancer Cells
Ting Chen 1ABCDEF , Chun Yang 2ABCDEFG* , Zhenli Xi 3ABDEF , Fen Chen 1ABCDE , Hailin Li 2ABCDEDOI: 10.12659/MSM.926443
Med Sci Monit 2020; 26:e926443
Figure 3 (A) Columns indicate the relative mRNA expression levels of dnmt1 and dnmt3a in SW480 cells incubated with curcumin at 0, 0.1, 0.2, or 0.4 μmol/L for 24 h. (B) Immunoblots of DNMT1, DNMT3a, and GAPDH. Columns indicate the relative expression levels of DNMT1 and DNMT3a in SW480 cells incubated with curcumin at 0, 0.1, 0.2, or 0.4 μmol/L for 24 h. (C) cdx2 promoter DNA methylation levels. Black dots indicate methylation sites while white dots indicate unmethylated cdx2 promoter sites. Columns indicate the DNA methylation rate of the cdx2 promoter in SW480 cells incubated with curcumin at 0, 0.1, 0.2, or 0.4 μmol/L for 24 h. (n=6, * P<0.05)