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07 July 2020: Animal Study

Baicalin Suppresses Bilirubin-Induced Apoptosis and Inflammation by Regulating p38 Mitogen-Activated Protein Kinases (MAPK) Signaling in Neonatal Neurons

Shuang Shi 1ABCDEF* , Qianwei Cui 1ACE* , Jing Xu 1CDEF , Zhiguo Tang 1ABC , Binya Shi 2ABCDEF* , Zhongwei Liu 1ABCDEFG*

DOI: 10.12659/MSM.926441

Med Sci Monit 2020; 26:e926441

Figure 5 (A) p65 was tagged with green fluorescence (Alexa488) and nuclei were tagged with blue fluorescence (DAPI). Columns indicated the nuclear translocation rate of p65 in Bf-incubated neurons treated with BAI at concentrations of 0, 0.5, 1.0, and 2.0 μmol/L. (B) The immunoblots of p-MKK3, MKK3, p-MKK6, MKK6, p-p38, and p38 of neurons were demonstrated. Columns indicate the relative phosphorylation levels of MKK3, MKK6, and p38. (C) Immunoblots of c-caspase3, TNF-α, and GAPDH of neurons. Columns indicate the relative expression levels of c-caspase3 and TNF-α in neurons. (D) Columns indicate the concentrations of TNF-α in cell medium of neurons. [n=10; a difference was significant when compared with neurons incubated with Bf at 280 nmol/L and BAI at 0 μmol/L; b difference was significant when compared with neurons incubated with Bf at 280 nmol/L and BAI at 0.5 μmol/L; c difference was significant when compared with neurons incubated with Bf at 280 nmol/L and BAI at 1.0 μmol/L].

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750