Figure 5 The expression of MUC15 in PC cell lines and transient transductions of MUC15 overexpression and knockdown were established. (A) MUC15 protein expression 6 PC cells lines. (B) The level of MUC15 mRNA in 6 PC cells lines. (C) Localization of MUC15 in cells by immunofluorescent analysis. White bar, 40 μm. (D, E) The efficiency of MUC15 overexpression and MUC15 knockdown in MIA PaCa-2 and Aspc-1 were confirmed by western blot and qRT-PCR. The data are presented as the mean±SD. (t test; *** p<0.001; **** p<0.0001) (MUC15-OE – MUC15 overexpression; MUC15-NC – MUC15 negative control; siMUC15 – MUC15 knockdown; siNC – scramble control).