05 October 2020: Animal Study
The Protein Kinase R Inhibitor C16 Alleviates Sepsis-Induced Acute Kidney Injury Through Modulation of the NF-κB and NLR Family Pyrin Domain-Containing 3 (NLPR3) Pyroptosis Signal Pathways
Jialu Zhou 1B* , Fan Zhang 2B* , Hongru Lin 1B , Minxue Quan 1B , Yaqin Yang 1B , Yanni Lv 3C , Zongnan He 4CE* , Yisong Qian 1ACE*DOI: 10.12659/MSM.926254
Med Sci Monit 2020; 26:e926254
Figure 5 C16 inhibits lipopolysaccharide (LPS)-induced pyroptosis after LPS challenge. (A) Immunoblots of apoptosis-associated speck-like protein (ASC); NACHT, LRR, NLR Family Pyrin Domain-Containing 3 (NLRP3); caspase-1 (cleaved form); and glyceraldehyde-3-phosphate dehydrogenase. We quantitatively analyzed levels of (B) ASC, (C) NLRP3, and (D) cleaved caspase-1. (E) The production of interleukin (IL)-1β in the kidney was detected with enzyme-linked immunosorbent assay (ELISA). (F) The production of IL-18 in the kidney was detected with ELISA. All values are shown as mean±standard deviation (n=8). ** P<0.01.