18 November 2020: Animal Study
Altered Inflammatory Pathway but Unaffected Liver Fibrosis in Mouse Models of Nonalcoholic Steatohepatitis Involving Interleukin-1 Receptor-Associated Kinase 1 Knockout
Ying Lei BCDE* , Tianxiao Yang BEFG* , Aijing Shan CDG , Wei Di BC , Mengyao Dai EFG , Jingminjie Nan B , Dongxue Liu B , Yanan Cao AEG , Xiuli Jiang AEG*DOI: 10.12659/MSM.926187
Med Sci Monit 2020; 26:e926187
Figure 4 Effects of Irak1 KO on collagen deposition in mouse models of NASH. (A, B) Representative Sirius red (upper part) and Masson (bottom part) stained liver sections of Irak1 KO and control mice following (A) CCl4 treatment for 8 weeks (n=5–6) or (B) MCDD feeding for 6 weeks (n=3–4). Scale bars, 100 μm. (C, D) Digital quantification of (C) Sirius red-positive and (D) Masson stain-positive areas of livers of Irak1 KO and control mice following CCl4 treatment for 8 weeks (n=5–6) or MCDD feeding for 6 weeks (n=3–4). (E, F) Hepatic levels of Sma, Col1a1, Col1a2, Timp1, Timp2, and Timp3 mRNAs in Irak1 KO and control mice following (E) CCl4 treatment for 8 weeks (n=5–6) or (F) MCDD feeding for 6 weeks (n=5). Data represent mean±SEM. * p<0.05 by t test.