24 July 2020>: Database Analysis
Systematic Analysis of Alternative Splicing Landscape in Pancreatic Adenocarcinoma Reveals Regulatory Network Associated with Tumorigenesis and Immune Response
Jiahua Lu 1234BCE , Shenyu Wei 5BCD , Jianying Lou 6BF , Shengyong Yin 1234DF , Lin Zhou 1234CD , Wu Zhang 78AEG* , Shusen Zheng 1234AEG*DOI: 10.12659/MSM.925733
Med Sci Monit 2020; 26:e925733
Figure 9 Consensus clustering based on prognosis-related alternative splicing (AS) events and immune landscape analysis. (A, B) Consensus clustering matrix and silhouette plot identifying 3 clusters. (C) Kaplan-Meier survival plot showing significant differences among the 3 clusters (log-rank t test, P<0.05). (D) Classification of pancreatic ductal carcinoma samples based on 5 immune subtypes in 3 clusters: wound healing, IFN-γ dominant, inflammatory, lymphocyte depleted, and TGF-β dominant. (E, F) Comparison of immune and stromal scores of the 3 clusters with analysis of variance. (G) Heat map of clinicopathological characteristics and gene phenotypes in the 3 clusters. Driver gene mutations, including KRAS, CDKN2A, and TP53, showed distinct distribution in the 3 clusters with chi-squared test.